RACGAP1/MgcRacGAP Antibody

SKU: orb18360

Description

Goat polyclonal antibody to RACGAP1

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration, 1ug/ml.WB: Approx 60kDa band observed in Human Testis lysates (calculated MW of 71.0kDa according to NP_037409.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Toure et al., J Biol Chem 1998 Mar 13;273(11):6019-23; PMID: 9497316). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RACGAP1 / MgcRacGAP
• Protein Sequence: GRQGNFFASPMLK
• Molecular Weight: 71.0; 63.9; 72.3; 61.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RACGAP1 antibody, anti MgcRacGAP antibody, anti Rac GTPase activating protein 1 antibody, anti ID-GAP antibody, anti MGCRACGAP antibody, anti GTPase activating protein antibody, anti HsCYK-4 antibody, anti HsCYK-4 antibody

Western blot analysis of Human Testis lysate using RACGAP1 antibody

Immunohistochemical staining of Human Testis using RACGAP1 antibody

Western blot analysis of HEK293 lysate using RACGAP1 antibody

Immunohistochemical staining of Human Testis using RACGAP1 antibody

Flow cytometric analysis of MCF7 cells using RACGAP1 antibody

Immunofluorescence analysis of MCF7 cells using RACGAP1 antibody

Quick Database Links

Gene Symbol

RACGAP1 / MgcRacGAP

NCBI Gene ID

29127