Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine/Human/goat/mouse/rat IgG

SKU: orb700289

Description

Goat polyclonal antibody against Goat anti-Rabbit IgG (H&L) conjugated to HRP

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: 1 : 200- 1 : 5 000 (ELISA), 1 : 500- 1 : 5 000 (IHC), 1 : 200- 1 : 5 000 (WB)
• Reactivity: Rabbit
• Application Notes:
  No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis,

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified Rabbit IgG, whole molecule (H&L chains)
• Purity: Immunogen affinity purified goat IgG.
• Purification: No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.No reactivity to non-immunoglobulin rabbit serum proteins.Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

Storage & Handling

• Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Form/Appearance: Lyophilized
• Disclaimer: For research use only