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Catalog Number | orb18329 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PPP2R5D |
Target | PPP2R5D |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | KRAEEFLTASQEAL |
RRID | AB_10750921 |
MW | 70.0; 66.2; 58.5; 53.1 |
Tested applications | ELISA, FC, IF, WB |
Dilution range | ELISA: 1:20000, WB: 1-3 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:20000.WB: Approx 70kDa and 60kDa bands seen in 293 lysate (Predicted MW of 68kDa and 62kDa according to NP_006236 and AAH01175, respectively). Recommended for use at 1-3 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PPP2R5D antibody, anti MGC2134 antibody, anti Read more... |
Note | For research use only |
Entrez | 5528 |
orb18329 staining (2ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.