PPP2R5D Rabbit Polyclonal Antibody

• Catalog Number: orb158192
• Category: Antibodies
• Description: PPP2R5D Rabbit Polyclonal Antibody
• Target: PPP2R5D
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human PPP2R5D (501-602/602aa)
• UniProt ID: Q14738
• MW: 70 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B'delta; delta isoform of regulatory subunit B56,
• Note: For research use only

Sample: Lane 1: mouse brain Lysate at 25 ug, Lane 2: mouse kidney Lysate at 25 ug, Primary: Anti-PPP2R5D (orb158192) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70kD, Observed band size: 78kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PPP2R5D Polyclonal Antibody, Unconjugated (orb158192) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.