PCNA (aa111-122) Antibody

SKU: orb43327

Description

Goat polyclonal to PCNA.Reported variants represent identical protein: NP_872590.1, NP_002583.1 .

Research Area

Cancer

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  WB: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calcu. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PCNA (aa111-122)
• Protein Sequence: EAPNQEKVSDYE
• Molecular Weight: 28.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration:

  0.5 mg/ml

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti cyclin antibody, DNA polymerase delta auxiliary protein antibody, MGC8367 antibody, OTTHUMP00000030189 antibody, OTTHUMP00000030190 antibody, PCNA antibody, proliferating cell nuclear antigen antibody

Western blot analysis of Jurkat lysate (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of Pig Spleen lysate using PCNA antibody

Immunohistochemical staining of Human Tonsil using PCNA antibody.

Quick Database Links

Gene Symbol

PCNA (aa111-122)

NCBI Gene ID

5111