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Catalog Number | orb403011 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PCNA |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 0.03-0.1 μg/ml |
Conjugation | Unconjugated |
MW | 28.8 |
Target | PCNA (aa111-122), Biotinylated |
Entrez | 5111 |
Protein Sequence | EAPNQEKVSDYE. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PCNA; proliferating cell nuclear antigen; MGC8367; Read more... |
Note | For research use only |
Application notes | Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates.WB: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calcu |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Jurkat lysate using PCNA antibody