P2X4/P2X4R Antibody

SKU: orb19982

Description

Goat polyclonal antibody to P2RX4

Research Area

Cardiovascular Disease, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:128000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 60-70kDa band observed in Human Kideney, Liver and Rat Kidney (calculated MW of 43.4kDa according to NP_002551.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Brown et al, J Biol Chem. 2004 Sep 17;279(38):39485-94.; PMID: 15262999). Recommended concentration: 1-3µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: P2X4 / P2X4R
• Protein Sequence: YREKKYKYVEDYEQ
• Molecular Weight: 43.4; 45.3; 40.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti P2X4 antibody, anti P2X4R antibody, anti P2RX4 antibody, anti purinergic receptor P2X, ligand-gated ion channel, 4 antibody, anti ATP receptor antibody, anti ATP-gated cation channel protein antibody, anti P2X purinoceptor 4 antibody, anti P2X receptor, subunit 4 antibody, anti purinergic receptor P2X ligand-gated ion channel 4 antibody, anti purinergic receptor P2X4 antibody, anti purinoceptor P2X4 antibody

Western blot analysis of Human Kidney lysate using P2RX4 antibody.

Flow cytometric analysis of paraformaldehyde fixed A431 cells using P2RX4 antibody.

Quick Database Links

Gene Symbol

P2X4 / P2X4R

NCBI Gene ID

5025