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P2RX4 Rabbit Polyclonal Antibody
SKU: orb100036
Featured
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human P2RX4 (238-338/388aa) |
| Target | P2RX4 |
| Molecular Weight | 43 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ATP gated cation channel protein; ATP receptor; p2rx4; P2RX4_HUMAN; P2X purinoceptor 4; P2X receptor subunit 4; P2X4; P2X4R; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 4; Purinergic receptor P2X4; Purinoceptor P2X4.
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Sample: Large intestine (Mouse) Lysate at 40 ug, Placenta (Mouse) Lysate at 40 ug, Primary: Anti-P2RX4 (orb100036) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.
Quick Database Links
Gene Symbol
P2RX4
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Ronald Sluyter 1, Tahnee B-D McEwan 2, Reece A Sophocleous 2, Leanne Stokes 3 Methods for studying P2X4 receptor ion channels in immune cells J Immunol Methods, 526, 113626 (2024)
Applications
Activity Assay
Aminin, Dmitry et al. Glycosides from edible sea cucumbers stimulate macrophages via purinergic receptors Sci Rep, 6, 39683 (2016)
Applications
FC
Reactivity
Mouse
P2RX4 Rabbit Polyclonal Antibody (orb100036)
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