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Catalog Number | orb154558 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to NOLC1 which is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins acts in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus and also participates in transcription catalyzed by RNA polymerase I. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes. |
Target | NOLC1 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Bovine, Canine, Mouse, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | QVNSIKFDSE |
MW | 73.6 |
Tested applications | ELISA, IHC |
Dilution range | ELISA: 1:16000, IHC-P: 2-4µg/ml |
Application notes | IHC: In paraffin embedded Human Kidney shows strong nucleolar staining in convoluted tubules. Recommended concentration, 2-4 μg/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti HCV NS5A trans-regulated protein 13 antibody, Read more... |
Note | For research use only |
Entrez | 9221 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Kidney using NOLC1 antibody
IHC, WB | |
Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Rabbit, Rat, Yeast | |
Human, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Drosophila, Human, Mouse, Plant, Rat | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |