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NMNAT3 Antibody

SKU: orb19870

Description

Goat polyclonal antibody to NMNAT3

Images & Validation

Tested ApplicationsELISA
Dilution rangeELISA: 1:32000, WB: 0.3-0.5 μg/ml, IHC-P: 10ug/ml
Predicted ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebral Cortex, Frontal Cortex, Hippocampus) lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.1kDa according to NP_835471.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetNMNAT3
Molecular Weight24.1; 18.3; 39.3; 28.3; 15.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti NMNAT3 antibody, anti nicotinamide nucleotide adenylyltransferase 3 antibody, anti PNAT-3 antibody, anti PNAT3 antibody, anti pyridine nucleotide adenylyltransferase 3 antibody

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NCBI Gene Details

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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NMNAT3 Antibody (orb19870)

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Available Sizes

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100 μg
$ 320.00
DispatchUsually dispatched within 5-10 working days
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