NMNAT3 (aa149-162) Antibody

SKU: orb125179

Description

Goat polyclonal antibody to NMNAT3

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  WB: Approx 26kDa band observed in Human Kidney lysates (calculated MW of 24.1kDa according to NP_835471.1). Recommended concentration: 0.1-0.3µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NMNAT3 (aa149-162)
• Protein Sequence: QHNIHLAKEPVQNE
• Molecular Weight: 24.1; 18.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NMNAT3 antibody, anti nicotinamide nucleotide adenylyltransferase 3 antibody, anti PNAT3 antibody, anti NMN adenylyltransferase 3 antibody, anti NaMN adenylyltransferase 3 antibody, anti nicotinamide mononucleotide adenylyltransferase 3 antibody, anti nicotinate-nucleotide adenylyltransferase 3 antibody, anti pyridine nucleotide adenylyltransferase 3 antibody, anti NaMN adenylyltransferase 3 antibody, anti nicotinamide mononucleotide adenylyltransferase 3 antibody, anti nicotinamide nucleotide adenylyltransferase 3 antibody, anti nicotinate-nucleotide adenylyltransferase 3 antibody, anti NMN adenylyltransferase 3 antibody, anti PNAT3 antibody, anti pyridine nucleotide adenylyltransferase 3 antibody, anti NMNAT3 antibody

Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

NMNAT3 (aa149-162)

NCBI Gene ID

349565