Myelin Protein zero Antibody

• Catalog Number: orb19823
• Category: Antibodies
• Description: Goat polyclonal antibody to MPZ
• Target: Myelin Protein zero
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DHSRSTKAVSEK
• RRID: AB_10751686
• MW: 28.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 28kDa band observed in Rat Spinal Cord lysates (calculated MW of 28.5kDa according to Human NP_000521.1 and of 27.7kDa according to Rat NP_058723.1). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti myelin protein zero antibody, anti myelin pro
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 4359

orb19823 (0.1ug/ml) staining of Rat Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.