Goat Anti-Mouse IgG (H&L) Antibody (HRP)

SKU: orb700404

Description

Goat polyclonal antibody against Goat anti-Mouse IgG (H&L) conjugated to HRP

Research Area

Cell Biology

Images & Validation

• Dilution Range: The optimal working dilution should be determined by the investigator, Suggested starting dilution: 1 : 20-1 : 2000 depending upon the application (immunolocalization or western blot)
• Reactivity: Mouse
• Application Notes:
  This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins as well as bovine, goat, human, rabbit or rat IgG.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Purification: Immunogen affinity purified IgG.
• Conjugation: HRP

Storage & Handling

• Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Form/Appearance: Lyophilized
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only