F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, HRP conjugated

• Catalog Number: orb1151905
• Category: Antibodies
• Description: F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, HRP conjugated
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Mouse
• Concentration: 1.0 mg/ml
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: Native Mouse IgG
• Tested applications: ELISA, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:1000, WB=1:1000-20000, IHC-F=1:100-1000, ELISA=1:1000-20000
• Antibody Type: Secondary Antibody
• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: F(ab)2 Fragment Goat Anti-Mouse IgG H&L (HRP); F(a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, An HRP-conjugated secondary (orb1151905, 1:300 dilution) was used for 20min at 37°C, DAB was used as the chromogen.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLP-1 (1G9)) Monoclonal Antibody, Unconjugated (orb10721) at 1:200 overnight at 4°C, An HRP-conjugated secondary (orb1151905, 1:300 dilution) was used for 20min at 37°C, DAB was used as the chromogen.