Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, HRP conjugated

• Catalog Number: orb700423
• Category: Antibodies
• Description: Goat polyclonal antibody against Goat anti-Mouse IgG (H&L) conjugated to HRP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: HRP
• Reactivity: Mouse
• Form/Appearance: Liquid
• Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
• Purification: This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
• Dilution range: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
• Application notes: This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
• Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Note: For research use only