MMP14 Antibody

SKU: orb107657

Description

Goat polyclonal antibody to MMP14

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  WB: Approx 60kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9kDa according to NP_004986.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MMP14
• Protein Sequence: ESATPLRFREVP
• Molecular Weight: 65.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MMP14 antibody, anti matrix metallopeptidase 14 (membrane-inserted) antibody, anti 1 antibody, anti MMP-14 antibody, anti MMP-X1 antibody, anti MT-MMP antibody, anti MT-MMP 1 antibody, anti MT1-MMP antibody, anti MT1MMP antibody, anti MTMMP1 antibody, anti matrix metalloproteinase 14 (membrane-inserted) antibody, anti matrix metalloproteinase-14 antibody, anti membrane type 1 metalloprotease antibody, anti membrane-type matrix metalloproteinase 1 antibody, anti membrane-type-1 matrix metalloproteinase antibody

Western blot analysis of A549 lysate (35ug protein in RIPA buffer) using MMP14 antibody

Western blot analysis of NIH3T3, (Lane 1) A549 (Lane 2) and HeLa (Lane 3) lysates using MMP14 antibody

Immunohistochemical staining of Human Liver using MMP14 antibody

Quick Database Links

Gene Symbol

MMP14

NCBI Gene ID

4323