MMP14 Antibody

• Catalog Number: orb107657
• Category: Antibodies
• Description: Goat polyclonal antibody to MMP14
• Target: MMP14
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESATPLRFREVP
• MW: 65.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 60kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9kDa according to NP_004986.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MMP14 antibody, anti matrix metallopeptidase
• Research Area: Cardiovascular Disease
• Note: For research use only
• Entrez: 4323
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A549 lysate (35ug protein in RIPA buffer) using MMP14 antibody

Western blot analysis of NIH3T3, (Lane 1) A549 (Lane 2) and HeLa (Lane 3) lysates using MMP14 antibody

Immunohistochemical staining of Human Liver using MMP14 antibody