MERTK Antibody

• Catalog Number: orb178368
• Category: Antibodies
• Description: Goat polyclonal antibody to MERTK.
• Target: MERTK
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EVHDSKPHEGRY
• MW: 110
• Tested applications: ELISA, FC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 125kDa band observed in lysates of cell lines THP-1 and A549 (calculated MW of 110.0kDa according to NP_006334.2). A 110kDa band was alternatively observed in some lysates. Recommended concentration: 2.5-3.5µg/ml. Primary incubation 1 hour at room temperature. Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti c-mer proto-oncogene tyrosine kinase antibody
• Note: For research use only
• Entrez: 10461

Primary incubation 1 hour at room temperature. Image A: THP-1 cell lysate at primary Ab concentration 3 ug/ml. Image B: A549 cell lysate at primary Ab concentration 3.5 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed THP1 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.