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Description
Research Area
Signal Transduction
Images & Validation
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Item 1 of 2
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 125kDa band observed in lysates of cell lines THP-1 and A549 (calculated MW of 110.0kDa according to NP_006334.2). A 110kDa band was alternatively observed in some lysates. Recommended concentration: 2.5-3.5µg/ml. Primary incubation 1 hour at room temperature. Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | MERTK |
| Protein Sequence | EVHDSKPHEGRY |
| Molecular Weight | 110 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti c-mer proto-oncogene tyrosine kinase antibody, anti MER antibody, anti MGC133349 antibody, anti c-mer antibody, anti MER receptor tyrosine kinase antibody, anti STK kinase antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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