MerTK Tyr749/753/754 Antibody

SKU: orb1401948

Description

Our Anti-MerTK (Tyr749/753/754) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects human, mouse, and rat MerTK Tyr749/753/754 and is antigen affinity purified from pooled serum.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 0.736111111
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Aves, Bovine, Canine, Gallus, Guinea pig, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr749/753/754 of human MerTK, conjugated to keyhole limpet hemocyanin (KLH).
• Target: MerTK Tyr749/753/754
• Molecular Weight: 160
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

c MER antibody, c mer proto oncogene tyrosine kinase antibody, c-mer antibody, cMER antibody, cmer protooncogene tyrosine kinase antibody, Eyk antibody, MER antibody, MER receptor tyrosine kinase antibody, MERK antibody, MERPEN antibody, Mertk antibody, MERTK c-mer proto-oncogene tyrosine kinase antibody, MERTK_HUMAN antibody, MGC133349 antibody, nmf12 antibody, Nyk antibody, Proto oncogene tyrosine protein kinase MER antibody, Proto oncogene tyrosine protein kinase MER precursor antibody, Proto-oncogene c-Mer antibody, Receptor tyrosine kinase MerTK antibody, RP38 antibody, STK kinase antibody, Tyrosine-protein kinase Mer antibody

Immunoblot was performed on cell lines treated for 24 h with Abe (2 μm), Palbo (5 μm), and/or Sitra (2 μm). Protein levels were determined for phospho-AXL, phosho-MET, and phosho-MERTK (Cat. orb1401948).

Western blot image of cultured A673 and TC106 cells treated with varying concentrations of MRX-2843 for 1 hour. MERTk was immunoprecipitated and phosphorylated (denoted by p) and the total MERTK and phosphorylated MERTK (Cat. orb1401948, 1:1000) were detected by immunoblot.

Western blot image of human THP-1 cells treated with γ-Carboxylated Gas6 at a 10 nM concentration showing increased MerTK phosphorylation (Cat. orb1401948).

Western blot of HEK293 lysate showing specific immunolabeling of the ~160 kDa MerTK phosphorylated at Tyr749/753/754 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 60 minutes).

Quick Database Links

Gene Symbol

MerTK Tyr749/753/754

UniProt

Q12866