KIT/CD117 Antibody

SKU: orb137105

Description

Goat polyclonal antibody to c-Kit

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates. Have any further splice variants/modified forms been reported.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KIT / CD117
• Molecular Weight: 110; 109
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration:

  1-3 μg/ml

• Disclaimer: For research use only

Alternative Names

anti-C Kit antibody, anti-c-kit antibody, anti-CD 117 antibody, anti-CD117 antibody, anti-CD117 antigen antibody, anti-KIT antibody, anti-KIT oncogene antibody, anti-Mast cell growth factor receptor antibody, anti-Mast/stem cell growth factor receptor antibody, anti-Mast/stem cell growth factor receptor Kit antibody, anti-p145 c-kit antibody, anti-PBT antibody, anti-Piebald trait protein antibody, anti-Proto oncogene c Kit antibody, anti-Proto oncogene tyrosine protein kinase Kit antibody, anti-Proto-oncogene c-Kit antibody, anti-SCF Receptor antibody, anti-SCFR antibody, anti-soluble KIT variant 1 antibody, anti-Steel Factor Receptor antibody, anti-Stem cell factor receptor antibody, anti-tyrosine protein kinase Kit antibody, anti-Tyrosine-protein kinase Kit antibody

WB analysis of Human Hippocampus lysate (Lane 1) + peptide (Lane 2) using c-Kit antibody

Immunofluorescence analysis of HEK293 cells using c-Kit antibody

Immunofluorescence analysis of HeLa cells using c-Kit antibody

Flow cytometric analysis of MCF7 cells using c-Kit antibody

Immunohistochemical staining of Human Prostate using c- kit antibody

Quick Database Links

Gene Symbol

KIT / CD117

NCBI Gene ID

3815