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Catalog Number | orb19766 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IGF2BP2 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Mouse |
Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 66.1; 61.8 |
Target | IGF2BP2 |
Entrez | 10644 |
Protein Sequence | KQQEQKYPQGVASQR |
RRID | AB_10751910 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IGF2BP2 antibody, anti insulin-like growth fa Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19766 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing IGF2BP2 (RC205673) and probed with orb19766 (mock transfection in first lane), tested by Origene.