IGF2BP2 Antibody

• Catalog Number: orb19766
• Category: Antibodies
• Description: Goat polyclonal antibody to IGF2BP2
• Target: IGF2BP2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KQQEQKYPQGVASQR
• RRID: AB_10751910
• MW: 66.1; 61.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti IGF2BP2 antibody, anti insulin-like growth fa
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 10644

orb19766 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IGF2BP2 (RC205673) and probed with orb19766 (mock transfection in first lane), tested by Origene.