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IDH1 (yeast) Antibody

SKU: orb22508

Description

Goat Polyclonal to IDH1

Research Area

Infectious Disease & Virology; Metabolism Research

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:16000, WB: 0.3-1 μg/ml
ReactivitySaccharomyces
Predicted ReactivityYeast
Application Notes
Tested with Saccharomyces cerevisiae S288cWB: Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Data kindly provided by F. Reggiiori, University Medical Centre Utrecht, Netherlands. Recommended concentration: 0.3-1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetIDH1 (yeast)
Protein SequenceEPGSRHVGLDIKGQN
Molecular Weight39.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti -Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase antibody, anti-which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle antibody, anti-YNL037C antibody, anti-Idh1p antibody, anti-IDH1 antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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