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HNF4A Antibody
SKU: orb19044
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 2 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Canine, Porcine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | HNF4A |
| Protein Sequence | RLSKTLVDMDMADY |
| Molecular Weight | 51.6; 52.8; 46.6 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti HNF4A antibody, anti TCF antibody, anti HNF4 antibody, anti NR2A1 antibody, anti TCF14 antibody, anti HNF4a7 antibody, anti HNF4a8 antibody, anti HNF4a9 antibody, anti NR2A21 antibody, anti hepatocyte nuclear factor 4, alpha antibody, anti HNF4-alpha antibody, anti transcription factor-14 antibody, anti FLJ39654 antibody, anti HNF4alpha antibody, anti MODY antibody, anti MODY1 antibody, anti HNF4alpha10/11/12 antibody
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orb19044 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of Human Small Intestine using HNF4A antibody
Western blot analysis of Mouse Small Intestine lysate using HNF4A antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HNF4A Antibody (orb19044)
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