HNF4A Antibody

SKU: orb19044

Description

Goat polyclonal antibody to HNF4A

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 2 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 52kDa and 48kDa bands observed in lysates of Human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8kDa according to NP_000448.3 and 46.6kDa according to NP_849181.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HNF4A
• Protein Sequence: RLSKTLVDMDMADY
• Molecular Weight: 51.6; 52.8; 46.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HNF4A antibody, anti TCF antibody, anti HNF4 antibody, anti NR2A1 antibody, anti TCF14 antibody, anti HNF4a7 antibody, anti HNF4a8 antibody, anti HNF4a9 antibody, anti NR2A21 antibody, anti hepatocyte nuclear factor 4, alpha antibody, anti HNF4-alpha antibody, anti transcription factor-14 antibody, anti FLJ39654 antibody, anti HNF4alpha antibody, anti MODY antibody, anti MODY1 antibody, anti HNF4alpha10/11/12 antibody

orb19044 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using HNF4A antibody

Western blot analysis of Mouse Small Intestine lysate using HNF4A antibody

Quick Database Links

Gene Symbol

HNF4A

NCBI Gene ID

3172