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histamine H3 receptor Antibody

SKU: orb12287

Description

Goat polyclonal antibody to HRH3

Research Area

Pharmacology & Drug Discovery

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
WB: Approx 48kDa band observed in lysates of cell line A549 (calculated MW of 48.7kDa according to NP_009163.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.

Key Properties

ClonalityPolyclonal
Targethistamine H3 receptor
Protein SequenceSVTRAVSYRAQQGDT
Molecular Weight48.7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti G protein-coupled receptor 97 antibody, anti GPCR97 antibody, anti G-protein coupled receptor 97 antibody, anti H3R antibody, anti HH3R antibody, anti histamine H3 receptor antibody, anti histamine receptor H3 antibody, anti HRH3 antibody, anti OTTHUMP00000031459 antibody

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histamine H3 receptor Antibody

Western blot analysis of A549 lysate using HRH3 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

histamine H3 receptor Antibody (orb12287)

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Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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