Histamine H3 Receptor Rabbit Polyclonal Antibody

SKU: orb224078

Description

The Histamine H3 Receptor Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H3 Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H3 Receptor. The exact sequence is proprietary.
• Target: HRH3
• Molecular Weight: 48 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPCR97; Histamine H3 receptor; H3R; HH3R; G-protein coupled receptor 97

Immunofluorescent analysis of Histamine H3 Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of paraffin-embedded mouse brain using Histamine H3 Receptor Antibody orb224078 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat stomach using Histamine H3 Receptor Antibody orb224078 with dilution at 1:100.

Western blot analysis of 293T (1), Hela (2) using Histamine H3 Receptor Antibody orb224078 with dilution at 1:1000.

Quick Database Links

Gene Symbol

HRH3

UniProt

Q9QYN8, P58406, Q9Y5N1

NCBI Gene ID

85268, 11255, 99296