Goat anti-HAP1 Antibody

SKU: orb19376

Description

Goat polyclonal antibody to HAP1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in Human Brain (Frontal Cortex and Hippocampus) lysates (calculated MW of 69.6kDa according to NP_817084.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HAP1
• Protein Sequence: RYDFRYSEDREQ
• Molecular Weight: 75.5; 69.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HAP1 antibody, anti huntingtin-associated protein 1 (neuroan 1) antibody, anti HAP2 antibody, anti HIP5 antibody, anti HLP antibody, anti hHLP1 antibody, anti huntingtin-associated protein 1 antibody, anti huntingtin-associated protein 2 antibody, anti neuroan 1 antibody

orb19376 (0.1ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Cortex using HAP1 antibody

Quick Database Links

Gene Symbol

HAP1

NCBI Gene ID

9001