Goat anti-Hap1 (mouse) Antibody

SKU: orb19482

Description

Goat polyclonal antibody to Hap1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 110kDa band in Mouse and Rat Brain lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.8kDa according to NP_034534.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Hap1 (mouse)
• Protein Sequence: QDAHSKRQQKQK
• Molecular Weight: 66.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Hap1 antibody, anti huntingtin-associated protein 1 antibody, anti RP23-392I3.11, HAP-1, MGC31449 antibody

Western blot analysis of Mouse Brain lysate using Hap1 antibody

Quick Database Links

Gene Symbol

Hap1 (mouse)