GPR125 Antibody

SKU: orb19860

Description

Goat polyclonal antibody to GPR125

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.5-1.5 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GPR125
• Molecular Weight: 146
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti GPR125 antibody, anti G protein-coupled receptor 125 antibody, anti PGR21 antibody, anti TEM5L antibody, anti TEM5-like antibody

Western blot analysis of Human Ovary lysate using GPR125 antibody

Immunohistochemical staining of Human Cortex using GPR125 antibody

Quick Database Links

Gene Symbol

GPR125

NCBI Gene ID

166647