Goat anti-GPR125 Antibody

• Catalog Number: orb19860
• Category: Antibodies
• Description: Goat polyclonal antibody to GPR125
• Target: GPR125
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CTNKSASSFKNS
• RRID: AB_10753585
• MW: 146
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.5-1.5 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GPR125 antibody, anti G protein-coupled recep
• Note: For research use only
• Entrez: 166647

Western blot analysis of Human Ovary lysate using GPR125 antibody

Immunohistochemical staining of Human Cortex using GPR125 antibody