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ADGRA3/GPR125 Antibody
Catalog Number: orb1539301
| Catalog Number | orb1539301 |
|---|---|
| Category | Antibodies |
| Description | ADGRA3/GPR125 Antibody |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, IHC-P, WB |
| Reactivity | Bovine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Rabbit |
| Immunogen | Peptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2. |
| Concentration | 0.5 mg/ml |
| Dilution range | IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:8000), WB (0.5 - 1.5 µg/ml) |
| Conjugation | Unconjugated |
| Target | ADGRA3 / GPR125 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Alternative names | ADGRA3, GPR125, TEM5L, G protein-coupled receptor Read more... |
| Note | For research use only |
| Application notes | Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2). Recommended concentration: 0.5-1.5 ug/ml. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported? |
| Expiration Date | 12 months from date of receipt. |
ADGRA3 / GPR125 antibody (0.5 µg/ml) staining of Human Ovary lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
