ADGRA3/GPR125 Antibody

• Catalog Number: orb1539301
• Category: Antibodies
• Description: ADGRA3/GPR125 Antibody
• Target: ADGRA3 / GPR125
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Rabbit
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:8000), WB (0.5 - 1.5 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2). Recommended concentration: 0.5-1.5 ug/ml. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ADGRA3, GPR125, TEM5L, G protein-coupled receptor
• Note: For research use only

ADGRA3 / GPR125 antibody (0.5 µg/ml) staining of Human Ovary lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.