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ADGRA3/GPR125 Antibody

SKU: orb1539301

Description

ADGRA3/GPR125 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:8000), WB (0.5 - 1.5 µg/ml)
ReactivityBovine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Rabbit
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2). Recommended concentration: 0.5-1.5 ug/ml. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2.
TargetADGRA3 / GPR125
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ADGRA3, GPR125, TEM5L, G protein-coupled receptor 125, PGR21

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ADGRA3/GPR125 Antibody

ADGRA3 / GPR125 antibody (0.5 µg/ml) staining of Human Ovary lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ADGRA3/GPR125 Antibody (orb1539301)

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Available Sizes

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50 μg
$ 670.00
DispatchUsually dispatched within 2-3 weeks
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