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| Catalog Number | orb154561 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to GLP1R, which belongs to the G-protein coupled receptor 2 family. GLP-1R expression primarily localizes to areas of the hypothalamus involved in feeding behavior. The activity of this receptor is mediated by G proteins which activates adenylyl cyclase. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior. |
| Target | GLP1R |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Porcine, Rat |
| Predicted Reactivity | Bovine, Canine, Mouse |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | QHQWDGLLSYQD |
| MW | 53 |
| Tested applications | ELISA, FC, IF, WB |
| Dilution range | ELISA: 1:128000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml |
| Application notes | WB: Approx 55kDa band observed in Human Brain (Cerebral and Frontal Cortex, Hippocampus) lysates (calculated MW of 53.0kDa according to NP_002053.3). Recommended concentration: 1-3 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-GLP 1 R antibody, anti-GLP 1 receptor antibod Read more... |
| Research Area | Neuroscience |
| Note | For research use only |
| Entrez | 2740 |

Western blot analysis of Human Hippocampus lysate using GLP1R antibody

WB analysis of Rat (A) and Pig (B) Brain lysate using GLP1R antibody

Immunofluorescence analysis of HeLa cells using GLP1R antibody

Flow cytometric analysis of HeLa cells using GLP1R antibody
IF, IHC-Fr, IHC-P, WB | |
Canine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.16-10 ng/mL | |
0.056 ng/mL |
Mouse | |
0.16-10 ng/mL | |
0.054 ng/mL |
Rat | |
0.16-10 ng/mL | |
0.057 ng/mL |
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