GLP1R Antibody

• Catalog Number: orb1248302
• Category: Antibodies
• Description: GLP1R Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Predicted Reactivity: Bovine, Canine, Mouse
• Reactivity: Human, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-QHQWDGLLSYQD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Hippocampus and in Rat and Pig Brain lysates (calculated MW of 53.0kDa according to Human NP_002053.3, 52.9kDa according to Rat NP_036860.1 and 52.7kDa according to Pig NP_001243523.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 55kDa
• Target: GLP1R
• UniProt ID: P43220
• NCBI: NP_002053.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Glp1r, glucagon-like peptide 1 receptor, GLP-1R, G
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Hippocampus and in Rat and Pig Brain lysates (calculated MW of 53.0kDa according to Human NP_002053.3, 52.9kDa according to Rat NP_036860.1 and 52.7kDa according to Pig NP_001243523.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248302 (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248302 (1 ug/ml) staining of Rat (A) and (0.1 ug/ml) Pig (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248302 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue).

orb1248302 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).