GCC185 Antibody

SKU: orb19848

Description

Goat polyclonal antibody to GCC2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:16000, WB: 0.03 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 60kDa band in Human Lymph Node and Testis lysates after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kDa according to NP_055450.1 and of 196kDa according to NP_852118.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GCC185
• Protein Sequence: QKLTNKNNKIED
• Molecular Weight: 185; 196
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GCC2 antibody, anti GRIP and coiled-coil domain containing 2 antibody, anti GCC185 antibody, anti KIAA0336 antibody, anti CLL-associated antigen KW-11 antibody, anti CTCL tumor antigen se1-1 antibody, anti GRIP and coiled-coil domain-containing 2 antibody, anti GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4) antibody

Immunohistochemical staining of Human Spleen using GCC2 antibody

Quick Database Links

Gene Symbol

GCC185

NCBI Gene ID

9648