Goat anti-GCC185 Antibody

• Catalog Number: orb19848
• Category: Antibodies
• Description: Goat polyclonal antibody to GCC2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human, Rat
• Dilution range: ELISA: 1:16000, WB: 0.03 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 185; 196
• Target: GCC185
• Entrez: 9648
• Protein Sequence: QKLTNKNNKIED
• RRID: AB_10748261
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GCC2 antibody, anti GRIP and coiled-coil doma
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 60kDa band in Human Lymph Node and Testis lysates after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kDa according to NP_055450.1 and of 196kDa according to NP_852118.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Spleen using GCC2 antibody