GCC2 Antibody

SKU: orb1260234

Description

GCC2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).
• Target: GCC2
• Molecular Weight: Observed: 196kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

GCC2, GRIP and coiled-coil domain containing 2, GCC185, KIAA0336, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GRIP and coiled-coil domain-containing 2, GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4), RANBP2L4

Western blot analysis of extracts of various cell lines, using GCC2 antibody (orb1260234) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody (orb1260234) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody (orb1260234) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody (orb1260234) at dilution of 1:150 (40x lens).

Quick Database Links

Gene Symbol

GCC2

UniProt

Q8IWJ2