Goat anti-GATA1 Antibody

SKU: orb570252

Description

Goat polyclonal antibody to GATA1

Research Area

Cancer Biology; Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: P-ELISA: 1:32000, IF/ICC: 10 μg/ml, FACS: 10 ug/ml, WB: 1-3μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 50kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incubation with the i. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

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Key Properties

• Clonality: Polyclonal
• Target: GATA1
• Protein Sequence: PTTSTTVVAPLSS
• Molecular Weight: 42.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GATA1; GATA binding protein 1 (globin transcription factor 1); GF1; NFE1; ERYF1

Quick Database Links

Gene Symbol

GATA1

NCBI Gene ID

2623