Goat anti-GATA1 Antibody

• Catalog Number: orb570252
• Category: Antibodies
• Description: Goat polyclonal antibody to GATA1
• Target: GATA1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PTTSTTVVAPLSS
• MW: 42.8
• Tested applications: ELISA, FC, IF, WB
• Dilution range: P-ELISA: 1:32000, IF/ICC: 10 μg/ml, FACS: 10 ug/ml, WB: 1-3μg/ml
• Application notes: WB: Approx 50kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incubation with the i. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GATA1; GATA binding protein 1 (globin transcriptio
• Note: For research use only
• Entrez: 2623

Flow cytometric analysis of paraformaldehyde fixed K562 cells using GATA1 antibody.

Western blot analysis of K562 nuclear cell lysate (lane 1) + peptide (lane 2) and negative control Human Hippocampus (lane 3) lysate using GATA1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using GATA1 antibody.