Goat anti-GATA1 Antibody

SKU: orb570252

Description

Goat polyclonal antibody to GATA1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: P-ELISA: 1:32000, IF/ICC: 10 μg/ml, FACS: 10 ug/ml, WB: 1-3μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 50kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incubation with the i. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GATA1
• Protein Sequence: PTTSTTVVAPLSS
• Molecular Weight: 42.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GATA1; GATA binding protein 1 (globin transcription factor 1); GF1; NFE1; ERYF1

Flow cytometric analysis of paraformaldehyde fixed K562 cells using GATA1 antibody.

Western blot analysis of K562 nuclear cell lysate (lane 1) + peptide (lane 2) and negative control Human Hippocampus (lane 3) lysate using GATA1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using GATA1 antibody.

Quick Database Links

Gene Symbol

GATA1

NCBI Gene ID

2623