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Catalog Number | orb19125 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to GATA1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml |
Conjugation | Unconjugated |
MW | 42.8 |
Target | GATA1 |
Entrez | 2623 |
Protein Sequence | DAEAYRHSPVFQ |
RRID | AB_10750664 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti GATA1 antibody, anti GATA binding protein 1 ( Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 48kDa band observed in lysates of human liver, placenta and peripheral blood mononucleocytes (calculated MW of 42.8kDa according to NP_002040.1. Recommended concentration: 0.3-1.0 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of paraformaldehyde fixed K562 cells using GATA1 antibody.
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells using GATA1 antibody.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using GATA1 antibody.
Western blot analysis of K562 nuclear cell lysate (lane 1) and negative control Human Hippocampus (lane 2) lysate using GATA1 antibody.