GATA1 Antibody

SKU: orb19125

Description

Goat polyclonal antibody to GATA1

Research Area

Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 48kDa band observed in lysates of human liver, placenta and peripheral blood mononucleocytes (calculated MW of 42.8kDa according to NP_002040.1. Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GATA1
• Protein Sequence: DAEAYRHSPVFQ
• Molecular Weight: 42.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GATA1 antibody, anti GATA binding protein 1 (globin transcription factor 1) antibody, anti HGNC:4170 antibody, anti ERYF1 antibody, anti GF1 antibody, anti NFE1 antibody, anti GATA binding protein 1 antibody, anti GATA-binding protein 1 (globin transcription factor 1) antibody, anti erythrold transcription factor 1 antibody, anti globin transcription factor 1 antibody, anti transcription factor GATA1 antibody, anti GF-1 antibody, anti XLTT antibody, anti NF-E1 DNA-binding protein antibody, anti erythroid transcription factor 1 antibody

Flow cytometric analysis of paraformaldehyde fixed K562 cells using GATA1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells using GATA1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using GATA1 antibody.

Western blot analysis of K562 nuclear cell lysate (lane 1) and negative control Human Hippocampus (lane 2) lysate using GATA1 antibody.

Quick Database Links

Gene Symbol

GATA1

NCBI Gene ID

2623