Goat anti-FUS Antibody

SKU: orb1524087

Description

FUS Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Between 1 and 50
• Target: FUS
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

TLS; HNRNPP2; oncogene FUS; translocated in liposarcoma protein; oncogene TLS; FUS/ERG chimeric protein; RNA-binding protein FUS; heterogeneous nuclear ribonucleoprotein P2; POMP75; fusion gene in myxoid liposarcoma; fused in sarcoma; FUS/ERG fusion protein; ETM4; ALS6; 75 kDa DNA-pairing protein; fus-like protein; FUS1

Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-FUS antibody orb1524087 (orb1524087) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-FUS antibody orb1524087 used for IP at 6 µg per reaction.

Detection of human FUS by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-FUS (Cat. No. orb1524087) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

FUS

UniProt

P35637

RefSeq (Protein):NP_004951.1