Goat anti-ERG Antibody

• Catalog Number: orb19134
• Category: Antibodies
• Description: Goat polyclonal antibody to ERG
• Target: ERG
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PNTRLPTSHMPSH
• RRID: AB_10751658
• MW: 53.8; 52.0; 54.6; 44.0
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Prostate shows nucleolar staining in some of the epithelial cells. Recommended concentration, 10-20ug/ml.WB: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-Avian erythroblastosis virus E-26 (v-ets) onc
• Research Area: Cancer, Epigenetics, Stem Cells
• Note: For research use only
• Entrez: 2078

Immunohistochemical staining of Human Prostate using ERG antibody

Western blot analysis of MOLT4 lysate using ERG antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using ERG antibody.

Immunofluorescence analysis of MCF7 cells using ERG antibody.