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Farnesoid X receptor/FXR Antibody

SKU: orb19387

Description

Goat polyclonal antibody to NR1H4

Research Area

Cancer

Images & Validation

Tested ApplicationsELISA, FC, IF
Dilution RangeELISA: 1:32000, WB: 1-3 μg/ml
ReactivityHuman
Predicted ReactivityBovine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in Human Duodenum and Human Lung lysates (calculated MW of 54.4kDa according to NP_005114.1). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetFarnesoid X receptor / FXR
Protein SequenceKSCREKTELTPDQQ
Molecular Weight54.4; 54.8; 56.0; 55.4; 48.8
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti farnesoid X receptor antibody, anti HRR1 antibody, anti NR1H4 antibody, anti nuclear receptor subfamily 1, group H, member 4 antibody, anti FXR antibody, anti HRR-1 antibody, anti RIP14 antibody, anti farnesol receptor HRR-1 antibody

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Farnesoid X receptor/FXR Antibody

orb19387 (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Farnesoid X receptor/FXR Antibody (orb19387)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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