NR1H4/FXR Antibody

• Catalog Number: orb1539468
• Category: Antibodies
• Description: NR1H4/FXR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Immunogen: Synthetic peptide located between aa194-243 of human NR1H4 (Q96RI1-2, NP_005114). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%); Hamster, Rabbit (92%); Turkey, Chicken (85%); Platypus (84%); M. tuberculosis (83%).
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: NR1H4 / FXR
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose
• Alternative names: NR1H4, BAR, Farnesyl x receptor, FXR, HRR1, Farnes
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B987 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B987 was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.