Ezrin/villin 2 Antibody

SKU: orb19239

Description

Goat polyclonal antibody to VIL2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Small Intestine shows strong staining of enterocytes. Recommended concentration, 3-5ug/ml.WB: Approx 80kDa band observed in Human Duodenum and Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Ezrin / villin 2
• Molecular Weight: 69.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti villin 2 (ezrin) antibody, anti ezrin antibody, anti VIL2 antibody, anti CVIL antibody, anti CVL antibody, anti DKFZp762H157 antibody, anti FLJ26216 antibody, anti MGC1584 antibody, anti cytovillin antibody, anti villin 2 antibody

Western blot analysis of Human Placenta lysate using VIL2 antibody

Immunohistochemical staining of paraffin embedded Human Small Intestine using VIL2 antibody

Immunohistochemical staining of Human Kidney using VIL2 antibody

Quick Database Links

Gene Symbol

Ezrin / villin 2

NCBI Gene ID

7430