Goat anti-Ezrin / villin 2 Antibody

• Catalog Number: orb19239
• Category: Antibodies
• Description: Goat polyclonal antibody to VIL2
• Target: Ezrin / villin 2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QDYEEKTKKAER
• RRID: AB_10750668
• MW: 69.4
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Small Intestine shows strong staining of enterocytes. Recommended concentration, 3-5ug/ml.WB: Approx 80kDa band observed in Human Duodenum and Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti villin 2 (ezrin) antibody, anti ezrin antibod
• Note: For research use only
• Entrez: 7430

Western blot analysis of Human Placenta lysate using VIL2 antibody

Immunohistochemical staining of paraffin embedded Human Small Intestine using VIL2 antibody

Immunohistochemical staining of Human Kidney using VIL2 antibody