EZR Antibody

• Catalog Number: orb1261790
• Category: Antibodies
• Description: EZR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human EZR (NP_003370.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 90kDa
• Target: EZR
• UniProt ID: P15311
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Ezrin, VIL2, villin 2 (ezrin), CVIL, CVL, DKFZp762
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EZR antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human stomach using EZR antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using EZR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using EZR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EZR antibody. Western blot was performed from the immunoprecipitate using EZR antibody at a dilition of 1:1000.