EZR Antibody

SKU: orb1261790

Description

EZR Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human EZR (NP_003370.2).
• Target: EZR
• Molecular Weight: Observed: 90kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Ezrin, VIL2, villin 2 (ezrin), CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, cytovillin, ezrin, villin 2

Western blot analysis of extracts of various cell lines, using EZR antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human stomach using EZR antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using EZR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using EZR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EZR antibody. Western blot was performed from the immunoprecipitate using EZR antibody at a dilition of 1:1000.

Quick Database Links

Gene Symbol

EZR

UniProt

P15311