ERN1/IRE1a Antibody

SKU: orb20044

Description

Goat polyclonal antibody to ERN1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 110kDa band observed in Human Pancreas lysates (calculated MW of 110kDa according to NP_001424.3). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: ERN1 / IRE1a
• Protein Sequence: PNAHGKIKAMISD
• Molecular Weight: 110
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ERN1 antibody, anti IRE1 antibody, anti endoplasmic reticulum to nucleus signaling 1 antibody, anti FLJ30999 antibody, anti IRE1P antibody, anti MGC163277 antibody, anti MGC163279 antibody, anti ER to nucleus signalling 1 antibody, anti endoplasmic reticulum to nucleus signalling 1 antibody, anti inositol-requiring 1 antibody, anti protein kinase/endoribonuclease antibody, anti IRE1a, IRE1-alpha antibody

Western blot analysis of Human Pancreas lysate using ERN1 antibody.

Flow cytometric analysis of HeLa cells using ERN1 antibody.

Quick Database Links

Gene Symbol

ERN1 / IRE1a

NCBI Gene ID

2081