Phospho-IRE1a (Ser 726) Rabbit Polyclonal Antibody

• Catalog Number: orb157704
• Category: Antibodies
• Description: Phospho-IRE1a (Ser 726) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human IRE1a around the phosphorylation site of Ser726 SF(p-S)RR
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 107 kDa
• Target: ERN1
• UniProt ID: O75460
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: p-IRE1a(phospho-Ser 726); IRE1(phospho S726); IRE1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mustafa Oztatlici,1 Hulya Oztatlici,2 Suna Karadeniz Saygili,3 Kaya Kaya,4 and Ilker Deniz Cingoz4 Cyclophosphamide stimulates endoplasmic reticulum stress and induces apoptotic cell death in human glioblastoma cell lines Rom J Morphol Embryol, (2024)

PubMed: 38527981

Applications

IHC

Reactivity

Human

Blank control: Raji. Primary Antibody (green line): Rabbit Anti-phospho-IRE1a (Ser 726) antibody (orb157704), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRE1a (Ser 726)) Polyclonal Antibody, Unconjugated (orb157704) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.