EPS8 Antibody

SKU: orb18755

Description

Goat polyclonal to EPS8

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:8000, IHC-P: 5-10µg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Breast Carcinoma shows staining of cancer cells infiltrated in a breast lobe. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: EPS8
• Protein Sequence: SGVESFDEGSSH
• Molecular Weight: 91.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EPS8 antibody, anti epidermal growth factor receptor pathway substrate 8 antibody

orb18755 (5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

EPS8

NCBI Gene ID

2059