EPS8 Antibody (N-term)

• Catalog Number: orb1928557
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB4003
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:2000, WB: 1:2000, WB: 1:500, WB: 1:1000, IHC-P: 1:50~100, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 91882 Da
• Target: This EPS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human EPS8.
• UniProt ID: Q12929
• NCBI: NP_004438.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Epidermal growth factor receptor kinase substrate
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-EPS8 Antibody (N-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: LOVO whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2OS cells labeling EPS8 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrance, cytoplasm weakly staining on U-2OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).

Overlay histogram showing U-2 OS cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

The anti-EPS8 Pab is used in Western blot to detect EPS8 in A375 cell lysate.

Anti-EPS8 Antibody (N-term) at 1:500 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-EPS8 Antibody (N-term) at 1:2000 dilution + LOVO whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.