Anti-EN2 Antibody

• Catalog Number: orb1879525
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EN2
• Clonality: Polyclonal
• Tested applications: FC, IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human EN2. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 13799, 2020
• UniProt ID: P19622, P09066
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: Homeobox protein engrailed-2; Homeobox protein en-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EN2 expression in mouse cerebellum (A), mouse brain (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 37 kD)

Immunohistochemical analysis of EN2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of K562 cells using Anti-EN2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.