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EEF1E1/AIMP3/p18 Antibody

SKU: orb137173

Description

Goat polyclonal to EEF1E1

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Porcine
Predicted ReactivityBovine, Canine
Application Notes
WB: Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3µg/ml. Primary incub. IHC: Paraffin embedded Human Pancreas. Recommended concentration: 5µg/ml.

Key Properties

ClonalityPolyclonal
TargetEEF1E1 / AIMP3 / p18
Protein SequenceSKGNKYSAQGERQ
Molecular Weight19.8; 15.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti EEF1E1 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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