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EEF1E1/AIMP3/p18 Antibody

SKU: orb1248347

Description

EEF1E1/AIMP3/p18 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Porcine
Predicted ReactivityBovine, Canine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-SKGNKYSAQGERQ
TargetEEF1E1 / AIMP3 / p18
Molecular WeightApprox 19kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

EEF1E1, eukaryotic translation elongation factor 1 epsilon 1, AIMP3, P18, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, eukaryotic translation elongation factor 1 epsilon-1

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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