E2F7 Antibody

SKU: orb20204

Description

Goat polyclonal antibody to E2F7

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 100kDa band observed in Human Brain (Cerebellum), Kidney and Testis lysates (calculated MW of 99.9kDa according to NP_976328.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: E2F7
• Protein Sequence: DLSKQKKFTPERNP
• Molecular Weight: 99.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti E2F7 antibody, anti E2F transcription factor 7 antibody, anti FLJ12981 antibody

orb20204 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

E2F7

NCBI Gene ID

144455