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E2f7 Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of mouse E2f7 |
| Target | E2f7 |
| Molecular Weight | 99kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−E2F7 Rabbit Polyclonal Antibody [orb221403]
ELISA, ICC, IF, IHC-Fr, IHC-P
Canine, Equine, Gallus, Human, Mouse, Rabbit, Rat, Sheep
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 200 μl, 50 μlE2F7 Rabbit Polyclonal Antibody [orb329926]
WB
Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat
Mouse
Rabbit
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100 μlE2f7 Rabbit Polyclonal Antibody [orb330518]
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Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Zebrafish
Rat
Rabbit
Polyclonal
Unconjugated
100 μl

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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Note this peptide is 85% identical to the human E2F7 sequence. A 76 kDa isoform also has an 85% match to this sequence.

Sample Tissue: Mouse Heart, Antibody Dilution: 1 ug/mL.

Sample Tissue: Mouse Liver, Antibody Dilution: 1 ug/mL.

Sample Tissue: NIH-3T3, Antibody Dilution: 1.0 ug/mL.

Sample Type: SP2/0 Whole Cell lysates, Antibody Dilution: 0.05 ug/mL.

Mouse stomach

WB Suggested Anti-E2f7 Antibody Titration: 0.2-1 ug/mL, Positive Control: SP2/0 cell lysate.
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Protocol Information
E2f7 Rabbit Polyclonal Antibody (orb329925)
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