Goat anti-Dynactin Antibody

• Catalog Number: orb18680
• Category: Antibodies
• Description: Goat polyclonal antibody to DCTN1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: 142; 127; 139; 127, 136, 140
• Target: Dynactin
• Entrez: 1639
• Protein Sequence: QEQLHQLHSRLIS
• RRID: AB_10767129
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DCTN1 antibody, anti dynactin 1 (p150, glued
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml.WB: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18680 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18680 (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.