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Dynactin Antibody

SKU: orb18680

Description

Goat polyclonal antibody to DCTN1

Research Area

Epigenetics, Neuroscience

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 2-4 μg/ml
ReactivityHuman
Predicted ReactivityMouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml.WB: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2 μg/ml.

Key Properties

ClonalityPolyclonal
TargetDynactin
Protein SequenceQEQLHQLHSRLIS
Molecular Weight142; 127; 139; 127, 136, 140
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti DCTN1 antibody, anti dynactin 1 (p150, glued homolog, Drosophila) antibody, anti P135 antibody, anti DP-150 antibody, anti DAP-150 antibody, anti p150-glued antibody, anti p150, Glued (Drosophila) homolog antibody, anti 150 kDa dynein-associated polypeptide antibody, anti dynactin 1 (p150, Glued (Drosophila) homolog) antibody, anti HMN7B antibody, anti OTTHUMP00000202491 antibody, anti dynactin 1 antibody, anti p150, Glued homolog antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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