Dynactin Antibody

SKU: orb18680

Description

Goat polyclonal antibody to DCTN1

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 2-4 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml.WB: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Dynactin
• Protein Sequence: QEQLHQLHSRLIS
• Molecular Weight: 142; 127; 139; 127, 136, 140
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DCTN1 antibody, anti dynactin 1 (p150, glued homolog, Drosophila) antibody, anti P135 antibody, anti DP-150 antibody, anti DAP-150 antibody, anti p150-glued antibody, anti p150, Glued (Drosophila) homolog antibody, anti 150 kDa dynein-associated polypeptide antibody, anti dynactin 1 (p150, Glued (Drosophila) homolog) antibody, anti HMN7B antibody, anti OTTHUMP00000202491 antibody, anti dynactin 1 antibody, anti p150, Glued homolog antibody

orb18680 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18680 (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Dynactin

NCBI Gene ID

1639